Coding

Part:BBa_K1998012:Design

Designed by: Shauna Winchester   Group: iGEM16_Macquarie_Australia   (2016-10-13)


HydEF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 253
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 903
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 304
    Illegal BsaI.rc site found at 416


Design Notes

Codon optimisation


Source

The genes were sourced from Chlamydomonas rienhardtii and synthesised.

References